mouse specific elisa kits  (R&D Systems)

Journal: Experimental Physiology

Article Title: Synergistic effect of vascular endothelial growth factor gene inactivation in endothelial cells and skeletal myofibres on muscle enzyme activity, capillary supply and endurance exercise in mice

doi: 10.1113/ep088924

Figure Lengend Snippet: Figure 1. Skeletal muscle VEGF levels. VEGF protein levels in the (A) soleus and (B) medial

Article Snippet: VEGF was measured using a mouse specific VEGF ELISA kit (R&D Systems, La Jolla, CA).

Techniques:

Journal: Experimental Physiology

Article Title: Synergistic effect of vascular endothelial growth factor gene inactivation in endothelial cells and skeletal myofibres on muscle enzyme activity, capillary supply and endurance exercise in mice

doi: 10.1113/ep088924

Figure Lengend Snippet: Figure 2. Treadmill exercise performance. WT, SkmVEGF-/-, EC-VEGF-/-, and Skm/EC-VEGF-/- mice

Article Snippet: VEGF was measured using a mouse specific VEGF ELISA kit (R&D Systems, La Jolla, CA).

Techniques:

Journal: Experimental Physiology

Article Title: Synergistic effect of vascular endothelial growth factor gene inactivation in endothelial cells and skeletal myofibres on muscle enzyme activity, capillary supply and endurance exercise in mice

doi: 10.1113/ep088924

Figure Lengend Snippet: Figure 3. Small Artery Number and Distribution is Maintatined in Skm/EC-VEGF-/- mice. (A) Total

Article Snippet: VEGF was measured using a mouse specific VEGF ELISA kit (R&D Systems, La Jolla, CA).

Techniques:

Journal: PLoS ONE

Article Title: Proliferative Activity of Liver Growth Factor is Associated with an Improvement of Cigarette Smoke-Induced Emphysema in Mice

doi: 10.1371/journal.pone.0112995

Figure Lengend Snippet: VEGF levels were estimated by ( A ) western blot and ( B ) ELISA. ( C ) PCNA levels estimated by western blot. Data were normalized with tubulin. ( D ) Representative images of PCNA immunohistochemistry staining in the nucleus is shown for CS-exposed mice (CSE) and CS-exposed and LGF-treated mice (CSE+LGF). Scale bars = 50 µm. LGF promoted an increase of PCNA+ cells as showed in the bar graph. ** P <0.01 vs. air-exposed mice; ¥ P <0.05 vs. CS-exposed mice (n = 5 per group). Data are presented as mean ± SEM.

Article Snippet: Using total protein extracts from lung tissue homogenates, ELISA was performed with a mouse specific VEGF ELISA kit (ref: CSB-E04756m; CUSABIO), as indicated in the manufacturer’s protocol.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Staining